15: PCR Protocols: Current Methods and Applications Ed. ^ Wojciech Rychlik (1993) Selection of Primers for Polymerase Chain Reaction, in Methods in Molecular Biology Vol.
#MACVECTOR SELECT INVERSE SOFTWARE#
^ "Oligo Primer Analysis Software from Molecular Biology Insights".Abd-Elsalam (2003) Bioinformatic Tools and Guideline for PCR Primer Design African Journal of Biotechnology 2, 91-95.
"Amplification of Nucleic Acids by Polymerase Chain Reaction (PCR) and Other Methods and Their Applications".
^ Bej AK, Mahbubani MH, Atlas RM (1991). Rhoads (1990) Optimization of the Annealing Temperature for DNA Amplification in Vitro Nucleic Acids Res. Rhoads (1989) A Computer Program for Choosing Optimal Oligonucleotides for Filter Hybridization, Sequencing and in vitro Amplification of DNA Nucleic Acids Research 17, 8543-8551. Offerman, Wojciech Rychlik (2003) Oligo Primer Analysis Software in Introduction to bioinformatics: a theoretical and practical approach. (2007) Basic Principles and Software for PCR Primer Design: Physical Principles and Visual-OMP Software for Optimal PCR Design, in Methods in Molecular Biology Vol. Oligo Primer Analysis Software has been used in various scientific studies (as cited by examples of recent publications), among them for: real time PCR, apoptosis studies, antigen typing, species identification, studies on species evolution, measuring mRNA expression levels, oligonucleotide-based array hybridization studies, degenerate primer studies, microsatellite analysis, DNA microarray detection, inverse PCR, genome walking, nucleotide polymorphisms studies, detection of microorganisms or viruses, genotyping, cloning, vector (gene) construction, genome sequencing, detection of mutants or intraspecific variability, genetic disease studies, siRNA and gene silencing, FISH analysis (single cell expression study), scorpion probes, and development of new DNA amplification methods. 
The OLIGO has been reviewed several times in scientific journals, for the first time in 1991 in a review in Critical Reviews in Biochemistry and Molecular Biology, and for its next upgrades (with OLIGO 7 being the latest one in 2011). It has been created and maintained by Wojciech Rychlik and Piotr Rychlik. The program is a comprehensive real time PCR primer and probe search and analysis tool, and also does other tasks such as siRNA and molecular beacon searches, open reading frame, restriction enzyme analysis, etc. The first papers describing this software were published in 19, and consecutive upgrades in the 1990s and 2000s, all have been cited together over 600 times in scientific journals and over 500 times in patents (according to Scopus). OLIGO Primer Analysis Software was the first publicly available software for DNA primer design.
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